Passage Number, *P4
Cell culture was started without cloning from the previous stock.
Date cell culture started:06/05/2002
Concentration of cells in an ampule: 9.6 x 10^5 cell/ml
Viability under microscope (%): 91.7
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, NP, AST, G6PD.
Marker chromosome: Majority was acrocentrics
Anchorage dependency: Yes
Cell Identification: available
RFLP data: STR-PCR experiments showed no peak. Chromosome analysis indeicated mouse cell line.

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal calf serum.
Freezing medium: Culture medium with 5% DMSO.
Passage method: trypsin-EDTA
Growth temperature: 37 C
CO₂ concentration: 5 %

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Freezing process,

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(Date downloaded: 2003-11-27)

Cells are propagated from the stock, 06192002
Passage Number, P7*
Cell culture was started without cloning from the previous stock.
Date cell culture started:07/08/2003
Concentration of cells in an ampule: 8.9 x 10^5 cell/ml
Viability under microscope (%): 88.1
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Anchorage dependency: Yes
Cell Identification: available
RFLP data: No peaks were apperared in PCR results that indicates the cells are not human.

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum.
Freezing medium: Culture medium containing 5% DMSO.
Passage method: Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 4.9 x 10^3 cells/sq.cm.
Additional comments: Mycoplasmas tested by PCR(Takada,Y.) method.

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Freezing process,

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(Date downloaded: 2003-11-27)