JCRB1032:OZ

JCRB1032 [OZ]

Profile:Human bile duct cell line established from ascites of the tumor patient who had differentiated adenocarcinoma.

Date accepted: 01/21/2003

Previous cell Number: NIHS0282

Animal: human

Sex and Age: M :71-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: Differentiated adenocarcinoma papilla and poorly differentiated adenocarcinoma. CEA 8.0 ng/ml.

Classification: tumor

Tissue prepared: ascites

Origin of tumor: bile dulct

Metastatic tissue: ascites

Date cell culture started: --/--/1981

History of the cell: Nagamori -> Okayama University -> JCRB

Medium: Williams'E medium with 10% fetal calf serum.

Passage method: Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.

CO₂ Concentration: 5 %

Genetics: No. of chromosome, 51. Td=48 hrs.

Life span: infinite

Morphology: epithelial-like

Characteristics: Cells were established from ascites of the patient whose bile duct was emphraxed by mucinous materials produced by adenocarcinoma cells. Cells produce CEA, BMG. High levels of P1-P, r-GTP, LDH were observed in cells. PAS (+). Transplantalbe to nude mice.

Cell ID data: available

DNA Profile: D5S818:13,14
D13S317:8,12
D7S820:10
D16S539:10
vWA:14,16
TH01:6,9
Amelogenin:XY
TPOX:9,11
CSF1PO:11,12

Virus DNA detected: GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested,GAPDH is positive control] -- Description

Established by Honma,S. & Nagamori,S.

Deposited by Nagamori,S.

Special notice: Require negotiation and agreement from Dr.Nagamori before shipping.

Cell bank: NIHS(JCRB)

References: (1)

Comments: Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. (r-GPT = gamma GPT)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

No extradocuments available.

[ ]

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 02212003: distribution (JCRB1032) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

96.1
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(not shown), NP, LDH examined. Human..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Williams'E medium with 10% fetal calf serum(Intergen RB52305).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.2% trypsin. Medium change twice a week and subculture every 10 days..

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.2 x 10^4 cells/sq.cm.

Memo:

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

Freezing process,

(Date downloaded: 2003-08-12)

[ Lot No.: 02202003: seed (JCRB1032) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

96.1
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB), NP, LDH examined. Human, not HeLa..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

William's E medium with 10 % fetal calf serum (Intergen RB52305).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells harvested after treatment with 0.2% trypsin. Subculture every 10 days and medium change twice a week. Cells attached hard to dishes and trypsinization at 37 C for 30 minutes required.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.4 x 10^6 cells/ml

Additional comments:

Mycoplasmas tested by staining(Kuretatsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2003-08-12)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1.Homma,S., Nagamori,S., Fujise,K., Yamazaki,K., Hasumura,S., Sujino,H., Matsuura,T., Shimizu,K., Kameda,H., and Takaki,K. Human bile duct carcinoma cell line producing abundant mucin in vitro, Gastroenterol Jpn., 22: 474-479, 1987.