JCRB9081 [L6]

Date accepted:11/21/86
Animal:rat
Scientific Name:Rattus norvegicus
Tissue:muscle
Medium:Dulbecco's modified Eagle's medium with 10 % fetal calf serum.
Passage method:Cells treated with 0.02% EDTA and 0.25% trypsin & subculture every 2-3 weeks.
Cell no. at passage:1/4 - 1/16
Genetics:skeletal muscle myoblast
Characteristics:skeletal muscle myoblast
Established byYaffe
Cell bank maintaining these cells.NIHS(JCRB)
References:(1-3)
Comments:Prepared from CRL1458.


[ Record(s) of stored ampules ]

[ Lot No.030287: token/seed(JCRB9081) ]
Date cell culture started:03/02/87
Concentration of cells in an ampule: 4x10^6 cell/ml
Culture medium: DMEM + FCS 10%
Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week.
Cell No. at passage: 1/4 - 1/16
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB showed original animals derived.
(Date downloaded: 1999-05-18)

[ Lot No.101188: DISTRIBUION(JCRB9081) ]
Date cell culture started:10/11/88
Concentration of cells in an ampule: 1.7x10^7 cell/ml
Culture medium: DMEM + FCS 10%
Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week.
Cell No. at passage: 1/4 - 1/16
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
(Date downloaded: 1999-05-18)

[ Lot No.092791: distribution(JCRB9081) ]
Date cell culture started:09/27/91
Concentration of cells in an ampule: 1.9x10^6 cell/ml
Culture medium: DMEM + FCS 10%
Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week.
Cell No. at passage: 1/4 - 1/16
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
(Date downloaded: 1999-05-18)

[ Lot No.051492: distribution(JCRB9081) ]
Date cell culture started:05/07/92
Concentration of cells in an ampule: 2.5x10^6 cell/ml
Culture medium: Dulbecco's EMEM(lot#.032592) + 10% FBS (Cell Culture Lab. lot#.00939-01)
Passage method: 0.25% trypsin in 0.02% EDTA, change medium or subculture every 3-4 days
Cell No. at passage: 1.03x10^6cells/10D.
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Anchorage dependency: Yes
Tumorgenesity: NY
Exogenous gene(s): NY
Memo: P22 printed to amp. is mistake. P8 is correct.
(Date downloaded: 1999-05-18)

[ Lot No.060796: distribution(JCRB9081) ]
Date cell culture started:05/30/96
Tests for mycoplasma, bacteria, and fungi were negative.
(Date downloaded: 1999-05-18)


Exported from cellroot.dbf and cellspec.dbf.

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