Date accepted: | 11/21/86 |
Animal: | rat |
Scientific Name: | Rattus norvegicus |
Tissue: | muscle |
Medium: | Dulbecco's modified Eagle's medium with 10 % fetal calf serum. |
Passage method: | Cells treated with 0.02% EDTA and 0.25% trypsin & subculture every 2-3 weeks. |
Cell no. at passage: | 1/4 - 1/16 |
Genetics: | skeletal muscle myoblast |
Characteristics: | skeletal muscle myoblast |
Established by | Yaffe |
Cell bank maintaining these cells. | NIHS(JCRB) |
References: | (1-3) |
Comments: | Prepared from CRL1458. |
Cell culture, Mycoplasma test, Isozyme analysis, Chromosome, Chromosome, Freezing process, Others(1), |
No extradocument. |
[ Lot No.030287: token/seed(JCRB9081) ] |
Date cell culture started:03/02/87 Concentration of cells in an ampule: 4x10^6 cell/ml Culture medium: DMEM + FCS 10% Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week. Cell No. at passage: 1/4 - 1/16 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB showed original animals derived. (Date downloaded: 1999-05-18) |
[ Lot No.101188: DISTRIBUION(JCRB9081) ] |
Date cell culture started:10/11/88 Concentration of cells in an ampule: 1.7x10^7 cell/ml Culture medium: DMEM + FCS 10% Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week. Cell No. at passage: 1/4 - 1/16 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. (Date downloaded: 1999-05-18) |
[ Lot No.092791: distribution(JCRB9081) ] |
Date cell culture started:09/27/91 Concentration of cells in an ampule: 1.9x10^6 cell/ml Culture medium: DMEM + FCS 10% Passage method: 0.02% EDTA and 0.25% trypsin. Every 2-3 week. Cell No. at passage: 1/4 - 1/16 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. (Date downloaded: 1999-05-18) |
[ Lot No.051492: distribution(JCRB9081) ] |
Date cell culture started:05/07/92 Concentration of cells in an ampule: 2.5x10^6 cell/ml Culture medium: Dulbecco's EMEM(lot#.032592) + 10% FBS (Cell Culture Lab. lot#.00939-01) Passage method: 0.25% trypsin in 0.02% EDTA, change medium or subculture every 3-4 days Cell No. at passage: 1.03x10^6cells/10D. Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes Tumorgenesity: NY Exogenous gene(s): NY Memo: P22 printed to amp. is mistake. P8 is correct. (Date downloaded: 1999-05-18) |
[ Lot No.060796: distribution(JCRB9081) ] |
Date cell culture started:05/30/96 Tests for mycoplasma, bacteria, and fungi were negative. (Date downloaded: 1999-05-18) |
2. Sato, H., Yoshida, M., and Sekiguchi, T. Q-banded karyotypes of a 6-thioguanine resistant rat cell line and its mutant line resistant to chloramphenicol. Chromosome Information Service, 27: 17-18, 1979. (Ref ID:1224).
3. Ohama, T., Muto, A., and Osawa, S. Spectinomycin operon of Micrococcus luteus: evolutionary implications of organization and novel codon usage. J. Mol. Evol., 29: 381-395, 1989. (Ref ID:866).