Reference for ES medium

• Koyama, H. and Kodama, H. Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FMA cells resistant to 4-carbamoylimidazolium 5-olate. Cancer Res. 42: 4210-4214, 1982.

  Notes : Reference for ES medium. 4-Carbamoylimidazolium 5-olate (CIO), the aglycone of the nucleoside antibiotic, bredinin (4-carbamoyl-1-beta-D-ribofuranosylimidazolium 5-olate), exhibited potent cytotoxic effects on subclonal line F28-7 of CH mouse mammary carcinoma FM3A cells in culture. We isolated 11 cell lines resistant to CIO from wild-type F28-7 cells mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine.....