BSS

代表的な Balanced Salt Solution (BSS) の組成


BSS(g/L)
試 薬 類Ringer (1895)SIMMS×7 (1942)Tyrode (1910)Earle (1943)Gey (1949)Hanks (1949)Rinaldini (1954)Puck (1958)Eagle* (1959)
NaCl8.08.08.06.88.08.08.08.06.8
KCl0.420.20.20.40.380.40.20.40.4
CaCl20.250.147(2H2O)0.20.20.310.140.20.0120.20(0.00*)
MgCl2・6H2O0.20.2030.1-0.21-0.012--
MgSO4---0.1-----
MgSO4・7H2O-----0.2-0.1540.2
NaH2PO4---0.125-----
NaH2PO4・H2O--0.050.125--0.05--
Na2HPO4・2H2O-0.213(-2H2O)---0.06--0.15(1.50*)
Na2HPO4・12H2O----0.3--0.39-
KH2PO4----0.0250.06-0.15-
Glucose-1.01.01.01.01.01.01.11.0
Na-citrate------0.676--
NaHCO3-1.011.02.20.250.351.0-2.0
Phenol red-0.05-0.05-0.02--0.05
AtmosphereAir5% CO2Air5% CO2AirAir5% CO2Air5% CO2


    * Eagle's BSS を改変したBSS.括弧内は接着性細胞を浮遊培養系に適応したときの処方.細胞数が少ない場合はNaHCO3を0.40gまで減らすこと.

Last Updated: Oct.31 1995 (att00006.htm, BSS)

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