JCRB1199 R201C

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1199 Cell Name   R201C
Profile   deficient human beta-gal introduced fibroblast (background, C57BL/6 beta-galactosidase KO mouse-derived SV cells) Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   4 days-old
Identity    Tissue for Primary Cancer   skin
Case history   mouse SV Cell line with a deficient behta-galactosidase mutated gene. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Namba,E. and Matsuda,J.
Registered by   Higaki,K. Regulation for Distribution   
Comment    Year   2005
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells were subcultured after treatment with standard trypsin-EDTA method.
Cell Number on Passage   Split ratio=1/3-1/4. Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6540
Reference
Pubmed id:14676316 Chemical chaperone therapy for brain pathology in G(M1)-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Yamamoto Y,Noguchi A,Takimoto K,Itoh M,Matsuzaki Y,Yasuda Y,Ogawa S,Sakata Y,Nanba E,Higaki K,Ogawa Y,Tominaga L,Ohno K,Iwasaki H,Watanabe H,Brady RO,Suzuki Y
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15912-7
Pubmed id:11504597 Galactonojirimycin derivatives restore mutant human beta-galactosidase activities expressed in fibroblasts from enzyme-deficient knockout mouse.
Tominaga L,Ogawa Y,Taniguchi M,Ohno K,Matsuda J,Oshima A,Suzuki Y,Nanba E
Brain Dev. 2001 Aug;23(5):284-7
Pubmed id:9337086 Beta-galactosidase-deficient mouse as an animal model for GM1-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Ogura A,Noguchi Y,Yamamoto Y,Asano T,Takimoto K,Sukegawa K,Suzuki Y,Naiki M
Glycoconj J. 1997 Sep;14(6):729-36

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LOT Information

Cell No.   JCRB1199 Cell Name   R201C
LOT No.   07072008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(Intergen RB52305) Temperature   37 C
Cell Density at Seeding   2.7x10^4 cells/sq.cm Methods for Passages   Cells were subcultured after treatment with standard 0.25% trypsin and 1mM EDTA method.No contact inhibition.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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