JCRB1312 rMSC-LacZ
Cell information
| JCRB No. | JCRB1312 | Cell Name | rMSC-LacZ |
|---|---|---|---|
| Profile | Mesenchymal stem cells from bone marrow of rat introduced with lacZ gene. | Other Name | |
| Animal | rat | Strain | Lewis |
| Genus | Rattus | Species | norvegicus |
| Sex | M | Age | adult |
| Identity | Tissue for Primary Cancer | bone marrow | |
| Case history | LacZ gene introduced | Metastasis | |
| Tissue Metastasized | Genetics | MHC haplotype RT1, LacZ gene introduced | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | transgenic | Established by | Inoue,S. et. al. |
| Registered by | Kobayashi,E. | Regulation for Distribution | Not applied. |
| Comment | Year | 2006 | |
| Medium | Mixed medium DMEM and F-12(1:1) with 10% fetal bovine serum. | Methods for Passages | Cells were removed by 0.25% tripsin in EDTA before subcultures. Keep 90% confluency when subculture. |
| Cell Number on Passage | Cell No at passage not defined | Race | |
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type | Mesenchymal stem cells |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:16770249 | Immunomodulatory effects of mesenchymal stem cells in a rat organ transplant model. Inoue S,Popp FC,Koehl GE,Piso P,Schlitt HJ,Geissler EK,Dahlke MH Transplantation. 2006 Jun 15;81(11):1589-95 |
| Pubmed id:15721305 | Development of new inbred transgenic strains of rats with LacZ or GFP. Inoue H,Ohsawa I,Murakami T,Kimura A,Hakamata Y,Sato Y,Kaneko T,Takahashi M,Okada T,Ozawa K,Francis J,Leone P,Kobayashi E Biochem Biophys Res Commun. 2005 Apr 1;329(1):288-95 |
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LOT Information
| Cell No. | JCRB1312 | Cell Name | rMSC-LacZ |
|---|---|---|---|
| LOT No. | 07042008 | Lot Specification | distribution |
| Medium | DMEM and F-12 mixed medium(1:1) with 10% heat inactivated bovine serum(Intergen Lot.RB52305) | Temperature | 37 C |
| Cell Density at Seeding | 1.5x10^4 cells/sq.cm | Methods for Passages | Cells were removed by 0.25% trypsin in EDTA before subculture.Keep 90% confluency when subculture. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.6x10^6 |
| Viability at cell freezing (%) | 95.2 | Antibiotics Used | free |
| Passage Number | p6 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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