JCRB1312 rMSC-LacZ

Cell information

JCRB No.   JCRB1312 Cell Name   rMSC-LacZ
Profile   Mesenchymal stem cells from bone marrow of rat introduced with lacZ gene. Other Name   
Animal   rat Strain   Lewis
Genus   Rattus Species   norvegicus
Sex   M Age   adult
Identity    Tissue for Primary Cancer   bone marrow
Case history   LacZ gene introduced Metastasis   
Tissue Metastasized    Genetics   MHC haplotype RT1, LacZ gene introduced
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transgenic Established by   Inoue,S. et. al.
Registered by   Kobayashi,E. Regulation for Distribution   Not applied.
Comment    Year   2006
Medium   Mixed medium DMEM and F-12(1:1) with 10% fetal bovine serum. Methods for Passages   Cells were removed by 0.25% tripsin in EDTA before subcultures. Keep 90% confluency when subculture.
Cell Number on Passage   Cell No at passage not defined Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   Mesenchymal stem cells

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8420
Reference
Pubmed id:16770249 Immunomodulatory effects of mesenchymal stem cells in a rat organ transplant model.
Inoue S,Popp FC,Koehl GE,Piso P,Schlitt HJ,Geissler EK,Dahlke MH
Transplantation. 2006 Jun 15;81(11):1589-95
Pubmed id:15721305 Development of new inbred transgenic strains of rats with LacZ or GFP.
Inoue H,Ohsawa I,Murakami T,Kimura A,Hakamata Y,Sato Y,Kaneko T,Takahashi M,Okada T,Ozawa K,Francis J,Leone P,Kobayashi E
Biochem Biophys Res Commun. 2005 Apr 1;329(1):288-95

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LOT Information

Cell No.   JCRB1312 Cell Name   rMSC-LacZ
LOT No.   07042008 Lot Specification   distribution
Medium   DMEM and F-12 mixed medium(1:1) with 10% heat inactivated bovine serum(Intergen Lot.RB52305) Temperature   37 C
Cell Density at Seeding   1.5x10^4 cells/sq.cm Methods for Passages   Cells were removed by 0.25% trypsin in EDTA before subculture.Keep 90% confluency when subculture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   p6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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