JCRB1685 HSC-3 CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines


Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1685 Cell Name   HSC-3 CMV-Luc
Profile   Luciferase stably expressing cell line (HSC-3;Human oral squamous carcinoma cell line with high metastatic potential cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   oral
Case history   squamous carcinoma, tongue. Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected HSC-3 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   squamous carcinoma, tongue, age 64, (M). Puromycin-resistant.
Classify   tumor Established by   
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells harvested after 0.25%trypsin and 0.02%EDTA treatment.Subculture with 1/8 dilution once a week.
Cell Number on Passage   1 x 10^5 cells/10cm dish Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JF94

LOT Information

Cell No.   JCRB1685 Cell Name   HSC-3 CMV-Luc
LOT No.   06282016 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.2-4.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. Subculture with 1/8 dilution once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.67x10^6
Viability at cell freezing (%)   100.0 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information