JCRB1774 KMP5-Luc#9

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

On the MTA of this cell line with Kyoto University (for profit organizations in Japan)

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

発光細胞 (ルシフェラーゼ発現細胞)の分譲について (KMPシリーズ細胞株)

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1774 Cell Name   KMP5-Luc#9
Profile   Luciferase stably expressing cell line (KMP5; human moderately differentiated adenocarcinoma cell line established from liver metastasis). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   55-year-old
Identity   not done Tissue for Primary Cancer   pancreas
Case history    Metastasis   Y
Tissue Metastasized    Genetics   pLVSIN-luc transfected. Luciferase stably expressing cell line. Moderately differentiated adenocarcinoma, stage 4.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Puromycin-resistant.
Classify   tumor Established by   Kato, M.
Registered by   Takashi Murakami Regulation for Distribution   
Comment    Year   
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   liver metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_XD22
Images
Movies



LOT Information

Cell No.   JCRB1774 Cell Name   KMP5-Luc#9
LOT No.   12132018 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(GIBCO)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.60-3.27x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.01x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11,13
D7S820:8,13
D16S539:9,11
VWA:18
TH01:7
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89
Additional information   
Images
CLOSE