JCRB3057 MK-4

Cell information

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB3057 Cell Name   MK-4
Profile   chromosome anomalad due to chromosome 21-trisomy Down's syndrome Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity    Tissue for Primary Cancer   
Case history   Down's syndrome Metastasis   
Tissue Metastasized    Genetics   48 XXY +21
Life Span    Crisis PDL   
Morphology    Character   
Classify   primary Established by   Sasaki M.
Registered by   Radiation Biology Center, Kyoto University Regulation for Distribution   
Comment    Year   
Medium    Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.    Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_XD26
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LOT Information

Cell No.   JCRB3057 Cell Name   MK-4
LOT No.   03252019 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) Temperature   37 C
Cell Density at Seeding   2.49-4.18x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.75x10^6
Viability at cell freezing (%)   99 Antibiotics Used   Plasmocure(Final concentration 50 ug/ml)
Passage Number   p5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,10
D7S820:11,12
D16S539:9,10
VWA:17,18
TH01:6,9
AM:X,Y
TPOX:11
CSF1PO:12,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   #03252019:Mycoplasma has eliminated with Plasmocure(Final concentration 50 ug/ml).
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Cell No.   JCRB3057 Cell Name   MK-4
LOT No.   03282019 Lot Specification   distribution2
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) Temperature   37 C
Cell Density at Seeding   2.26-3.68x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.79x10^6
Viability at cell freezing (%)   100 Antibiotics Used   Plasmocure(Final concentration 100 ug/ml)
Passage Number   p6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,10
D7S820:11,12
D16S539:9,10
VWA:17,18
TH01:6,9
AM:X,Y
TPOX:11
CSF1PO:12,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   #03282019:Mycoplasma has eliminated with Plasmocure(Final concentration 100 ug/ml).
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