JCRB3080 AT17BISV

Cell information

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB3080 Cell Name   AT17BISV
Profile   Hereditary disease with high cancer risk ataxia telangiectasia (autosomal recessive) chromosome breakage syndrome Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   Ataxia telangiectasia Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology   fibroblast-like Character   SV40 Transfection
Classify   transformed Established by   
Registered by   Radiation Biology Center, Kyoto University Regulation for Distribution   
Comment    Year   
Medium   MEM alpha with 10% fetal bovine serum (not HI) Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   
CO2 Conc.    Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

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LOT Information

Cell No.   JCRB3080 Cell Name   AT17BISV
LOT No.   08012023 Lot Specification   distribution
Medium   MEM alpha (Fujifilm WAKO: Cat#137-17215) with 10% fetal bovine serum (not HI) (SIGMA, Cat#172012; Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.5-3.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.07x10^6
Viability at cell freezing (%)   90.2 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,11
D7S820:10,11
D16S539:9,12
VWA:17
TH01:9,9.3
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker (Nihon Zenyaku Kogyo Co.Ltd : catalog No. CB011) CO2 Conc.   5%
Viability immediately after thawing (%)   78.2 Additional information   
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