JCRB4023 MEF

Cell information

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB4023 Cell Name   MEF
Profile   Mouse embryonic fibroblasts isolated from B6N albino mouse embryos are immortalized by expressing SV40 LargeT with a retroviral vector (pMSCV). (This cell line was established as the control of Blvra-/-MEF, but not for the use for feeder cells such as iPS cell culture.) Other Name   
Animal   mouse Strain   C57BL/6NCrl
Genus    Species   
Sex    Age   embryo
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Immortalized by SV40 large T via retroviral gene transfer (pMSCV). Puromycin-resistant.
Classify   transformed Established by   Kenju KOBACHI
Registered by   Michiyuki MATSUDA Regulation for Distribution   
Comment    Year   
Medium   DMEM (high-glucose) with 10% FBS Methods for Passages    Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:32581154Biliverdin Reductase-A Deficiency Brighten and Sensitize Biliverdin-binding Chromoproteins.
Kobachi K,Kuno S,Sato S,Sumiyama K,Matsuda M,Terai K
Cell Struct Funct. 2020 Aug 21;45(2):131-141




LOT Information

Cell No.   JCRB4023 Cell Name   MEF
LOT No.   04302024 Lot Specification   distribution
Medium   DMEM (4.5g/L D-Glucose, GIBCO Cat#11995-065) with 10% heat inactivated fetal bovine serum (SIGMA, Cat# 172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.263-1.50x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.85x10^6
Viability at cell freezing (%)   99.46 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   99.12 Additional information   
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