JCRB4023 MEF
Cell information
Cell type:genetically-modified cells (View Pricing Information)
JCRB No. | JCRB4023 | Cell Name | MEF |
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Profile | Mouse embryonic fibroblasts isolated from B6N albino mouse embryos are immortalized by expressing SV40 LargeT with a retroviral vector (pMSCV). (This cell line was established as the control of Blvra-/-MEF, but not for the use for feeder cells such as iPS cell culture.) | Other Name | |
Animal | mouse | Strain | C57BL/6NCrl |
Genus | Species | ||
Sex | Age | embryo | |
Identity | not done | Tissue for Primary Cancer | |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | fibroblast-like | Character | Immortalized by SV40 large T via retroviral gene transfer (pMSCV). Puromycin-resistant. |
Classify | transformed | Established by | Kenju KOBACHI |
Registered by | Michiyuki MATSUDA | Regulation for Distribution | |
Comment | Year | ||
Medium | DMEM (high-glucose) with 10% FBS | Methods for Passages | Trypsin-EDTA |
Cell Number on Passage | Race | ||
CO2 Conc. | 5% | Tissue Sampling | embryo |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:32581154 | Biliverdin Reductase-A Deficiency Brighten and Sensitize Biliverdin-binding Chromoproteins. Kobachi K,Kuno S,Sato S,Sumiyama K,Matsuda M,Terai K Cell Struct Funct. 2020 Aug 21;45(2):131-141 |
LOT Information
Cell No. | JCRB4023 | Cell Name | MEF |
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LOT No. | 04302024 | Lot Specification | distribution |
Medium | DMEM (4.5g/L D-Glucose, GIBCO Cat#11995-065) with 10% heat inactivated fetal bovine serum (SIGMA, Cat# 172012, Lot#12J396) | Temperature | 37 C |
Cell Density at Seeding | 0.263-1.50x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.85x10^6 |
Viability at cell freezing (%) | 99.46 | Antibiotics Used | free |
Passage Number | p3* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | |
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 99.12 | Additional information |