JCRB4025 BlvraKO MEF
Cell information
Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB4025 | Cell Name | BlvraKO MEF |
|---|---|---|---|
| Profile | Mouse embryonic fibroblasts isolated from B6N albino mouse fetuses with Blvra KO (Blvra-/-) immortalized by expressing SV40 LargeT by retroviral vector (pMSCV) | Other Name | |
| Animal | mouse | Strain | C57BL/6NCrl |
| Genus | Species | ||
| Sex | Age | embryo | |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Blvra KO（Blvra-/-） | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Immortalized by SV40 large T via retroviral gene transfer (pMSCV). Puromycin-resistant. |
| Classify | transformed | Established by | Kenju KOBACHI |
| Registered by | Michiyuki MATSUDA | Regulation for Distribution | |
| Comment | Year | ||
| Medium | DMEM (high-glucose) with 10% FBS | Methods for Passages | Trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:32581154 | Biliverdin Reductase-A Deficiency Brighten and Sensitize Biliverdin-binding Chromoproteins. Kobachi K,Kuno S,Sato S,Sumiyama K,Matsuda M,Terai K Cell Struct Funct. 2020 Aug 21;45(2):131-141 |
LOT Information
| Cell No. | JCRB4025 | Cell Name | BlvraKO MEF |
|---|---|---|---|
| LOT No. | 05202024 | Lot Specification | distribution |
| Medium | DMEM (4.5g/L D-Glucose, GIBCO Cat#11995-065) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 0.421-2.18 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.06x10^6 |
| Viability at cell freezing (%) | 100.00 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 83.23 | Additional information |