IFO50022 HL-60

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50022 Cell Name   HL-60
Profile   IFO strain exhibits more rapid growth compared to the original HL-60 strain. IFO strain does not respond to DMSO or TPA for differentiation unlike to original HL-60 strain. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   36
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   promyelocytic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   leukemia, acute promyelocytic, This strain is unable to induce differentiation. =JCRB0006 HL60RG
Classify   tumor Established by   Collins, S. J. et al.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment   This line is equivalent to HL60RG (JCRB 0006). Year   1985
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0002
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LOT Information

Cell No.   IFO50022 Cell Name   
LOT No.   11182002 Lot Specification   distribution
Medium   RPMI1640 with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2-3 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   29 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   74.1 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50022 Cell Name   HL-60
LOT No.   1056 Lot Specification   distribution
Medium   PRMI 1640 , 90% ; heat-inactivated FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50022 Cell Name   HL-60
LOT No.   02012023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   approx. 4-6 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   23-40 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.4
Additional information   
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