IFO50034 RBL-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50034 Cell Name   RBL-1
Profile   leukemia, basophilic (Wistar rat) Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   basophilic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   basophilic leukemia
Classify   tumor Established by   Eccleston, E. et al.
Registered by   Terao,S. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0496
Reference
Pubmed id:3237999Use of simplified substrates for the study of 5-lipoxygenase from RBL-1 cells.
Navé JF,Dulery B,Gaget C,Ducep JB
Prostaglandins. 1988 Sep;36(3):385-98
Pubmed id:4812630The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE.
Kulczycki A Jr,Isersky C,Metzger H
J Exp Med. 1974 Mar 1;139(3):600-16
Pubmed id:4516144Basophilic leukaemia in the albino rat and a demonstration of the basopoietin.
Eccleston E,Leonard BJ,Lowe JS,Welford HJ
Nat New Biol. 1973 Jul 18;244(133):73-6

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LOT Information

Cell No.   IFO50034 Cell Name   RBL-1
LOT No.   423 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50034 Cell Name   RBL-1
LOT No.   03292016 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx 4-6 x 10^5 cells/mL Methods for Passages   RBL-1 consisted of suspending cells and adherent cells. Both types of cells should be passaged. Adherent cells were detathed with 0.25% trypsin and 0.02% EDTA - PBS.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   5.8 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90
Additional information   
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