IFO50038 U-937

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50038 Cell Name   U-937
Profile   lymphoma, diffuse histiocytic, monocyte-like characteristics Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   37
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   histcytic lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   lymphoma, histiocytic
Classify   tumor Established by   Sundstrom, C. & Nilsson, K.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0007
Reference
Pubmed id:7381208 Chemotactically responsive and nonresposive forms of a continuous human monocyte cell line.
Fischer DG,Pike MC,Koren HS,Snyderman R
J Immunol. 1980 Jul;125(1):463-5
Pubmed id:178611 Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundström C,Nilsson K
Int J Cancer. 1976 May 15;17(5):565-77
Pubmed id:1083890 Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
Ralph P,Moore MA,Nilsson K
J Exp Med. 1976 Jun 1;143(6):1528-33

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LOT Information

Cell No.   IFO50038 Cell Name   
LOT No.   08162004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   ca. 31 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P7 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50038 Cell Name   U-937
LOT No.   331 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10,12
D7S820:9,11
D16S539:12
VWA:14,15
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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