IFO50040 Namalwa

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50040 Cell Name   Namalwa
Profile   This cell line produce interferon. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   Burkitt's lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   lymphoma, Burkitt's
Classify   tumor Established by   Singh, S., (biopsy taken by)
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lymphoblastoid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0067
Reference
Pubmed id:464569Large-scale production and concentration of human lymphoid interferon.
Klein F,Ricketts RT,Jones WI,DeArmon IA,Temple MJ,Zoon KC,Bridgen PJ
Antimicrob Agents Chemother. 1979 Mar;15(3):420-7
Pubmed id:4365093Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line.
Nyormoi O,Klein G,Adams A,Dombos L
Int J Cancer. 1973 Sep 15;12(2):396-408
Pubmed id:4122458Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus.
Klein G,Dombos L,Gothoskar B
Int J Cancer. 1972 Jul 15;10(1):44-57

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LOT Information

Cell No.   IFO50040 Cell Name   
LOT No.   07182008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   0.5 - 2 x 10^5 cells/mL Methods for Passages   dilution (suspension culture)
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   8.8 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:11
D16S539:9
VWA:14
TH01:7,9.3
AM:X
TPOX:6,11
CSF1PO:10,11
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50040 Cell Name   Namalwa
LOT No.   844 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^7
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50040 Cell Name   Namalwa
LOT No.   04032019 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 17 hrs. Cell Number in Vial (cells/1ml)   8.5 x 10^6
Viability at cell freezing (%)   86.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   85.9
Additional information   
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