IFO50043 WiDr

Cell information

Important Notice(s)

JCRB0224, IFO50043 WiDr is derivative from HT-29.

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50043 Cell Name   WiDr
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   78
Identity   available Tissue for Primary Cancer   colon
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   adenocarcinoma
Classify   tumor Established by   Wallace, R.
Registered by   Suzuki,N. Regulation for Distribution   
Comment   WiDr cell line is suggested to be derived from a human colon adenocarcinoma cell line HT-29 (Cancer Genet. Cytogenet., 27,125 (1987)) Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2760

LOT Information

Cell No.   IFO50043 Cell Name   WiDr
LOT No.   1160 Lot Specification   distribution
Medium   E.MEM with non-essential amino acids, 90%; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-1.5 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information