IFO50067 LoVo

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50067 Cell Name   LoVo
Profile   adenocarcinoma, carcinoembryonic antigen-producing Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56
Identity   available Tissue for Primary Cancer   colon
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   adenocarcinoma, carcinoembryonic antigen-producing
Classify   tumor Established by   Drewinko, B. et al.
Registered by   Kondo,K. Regulation for Distribution   
Comment    Year   1985
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0399
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LOT Information

Cell No.   IFO50067 Cell Name   LoVo
LOT No.   02172004 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS Lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.2 - 1.6 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 1.2 days Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12,13
D13S317:8,11
D7S820:9.3,10,11
D16S539:9,12
VWA:17,18
TH01:9.3
AM:X,Y
TPOX:8,9
CSF1PO:11,13,14
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50067 Cell Name   LoVo
LOT No.   415 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50067 Cell Name   LoVo
LOT No.   02132004 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS Lot. GIBCO 3762782S). Temperature   37 C
Cell Density at Seeding   6-8 x 10^4 cells/sq.cm. Methods for Passages   0.25 % trypsin and 0.02% EDTA.
Doubling Time   Ca. 1.2 days Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5 % DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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