IFO50071 MDCK (NBL-2)

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50071 Cell Name   MDCK (NBL-2)
Profile   Epitherial-like cell line established from normal kidney of dog, susceptible for many viral species. Other Name   MDCK
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   adult
Identity    Tissue for Primary Cancer   kidney
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   established from normal kidney, susceptible for many viral species
Classify   normal Established by   Madin, S. H. & Darby, N. B.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Virus Summary   tested [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0422
Images



LOT Information

Cell No.   IFO50071 Cell Name   
LOT No.   05242004 Lot Specification   distribution
Medium   EMEM + 10% FBS(FBS lot; GIBCO 3762782S) Temperature   37C
Cell Density at Seeding   8 x 10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   3 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as dog by NP, G6PD, AST, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   YES Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS, 70% EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50071 Cell Name   MDCK (NBL-2)
LOT No.   190 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, MPI, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50071 Cell Name   MDCK (NBL-2)
LOT No.   11242016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   8.7 - 9.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 15 min.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
CLOSE