IFO50072 MRC-9

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50072 Cell Name   MRC-9
Profile   Normal diploid fibroblasts with limited life span (crisis PDL = 44). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   15-week fetus
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   44
Morphology   fibroblast-like Character   fetus lung normal diploid fibroblast
Classify   primary Established by   Jacobs, J. P. et al.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2629
Reference
Pubmed id:113411 Characteristics of a serially propagated human diploid cell designated MRC-9.
Jacobs JP,Garrett AJ,Merton R
J Biol Stand. 1979 Apr;7(2):113-22

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LOT Information

Cell No.   IFO50072 Cell Name   
LOT No.   09182001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   7-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Growth at least for additional 23 PDL was confirmed at bank.
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50072 Cell Name   MRC-9
LOT No.   181 Lot Specification   seed/distribution
Medium   E.MEM, 90% FBS 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, MPI, PepB, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:10,11
D16S539:12
VWA:16,17
TH01:6,8
AM:X
TPOX:11
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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