IFO50108 MG-63

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50108 Cell Name   MG-63
Profile   osteosarcoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14
Identity   available Tissue for Primary Cancer   sarcoma
Case history   osteosarcoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal Character   osteosarcoma
Classify   tumor Established by   Billiau, A. et al.
Registered by   Igarashi,K. Regulation for Distribution   
Comment    Year   1986
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat inactivated FBS Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0426
Reference
Pubmed id:883813 Human interferon: mass production in a newly established cell line, MG-63.
Billiau A,Edy VG,Heremans H,Van Damme J,Desmyter J,Georgiades JA,De Somer P
Antimicrob Agents Chemother. 1977 Jul;12(1):11-5

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LOT Information

Cell No.   IFO50108 Cell Name   
LOT No.   03182011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   82.9 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:11
D7S820:10
D16S539:11,12
VWA:16,19
TH01:9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50108 Cell Name   
LOT No.   05262004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 1 day Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   86.4 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50108 Cell Name   MG-63
LOT No.   1032 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; heat-inactivated FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6 x 10^5
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50108 Cell Name   MG-63
LOT No.   451 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; heat-inactivated FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^5
Viability at cell freezing (%)   78 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50108 Cell Name   MG-63
LOT No.   05242018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.5 - 4.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87
Additional information   
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