IFO50153 A549

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50153 Cell Name   A549
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   adenocarcinoma
Classify   tumor Established by   Giard, D. J. et al.
Registered by   Suzuki,N. Regulation for Distribution   
Comment    Year   1987
Medium   Ham's F12K medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0023
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905949
Reference
Pubmed id:2783462 Inhibition of growth of human lung adenocarcinoma cell lines by anti-transforming growth factor-alpha monoclonal antibody.
Imanishi K,Yamaguchi K,Kuranami M,Kyo E,Hozumi T,Abe K
J Natl Cancer Inst. 1989 Feb 1;81(3):220-3
Pubmed id:175022 A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells.
Lieber M,Smith B,Szakal A,Nelson-Rees W,Todaro G
Int J Cancer. 1976 Jan 15;17(1):62-70
Pubmed id:4357758 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP
J Natl Cancer Inst. 1973 Nov;51(5):1417-23

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LOT Information

Cell No.   IFO50153 Cell Name   
LOT No.   01212005 Lot Specification   distribution
Medium   Ham's F12K medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.2 - 4.2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   40 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   unknown (P8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:11,12
VWA:14
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50153 Cell Name   A549
LOT No.   1087 Lot Specification   distribution
Medium   Ham's F12K, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6 x 10^5
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(typeB), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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