IFO50221 N18-RE-105

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50221 Cell Name   N18-RE-105
Profile   Neuron like cells established from mouse N18TG2 x Fischer rat 18 day-embryonic neural retina. Other Name   
Animal   mouse x rat Strain   
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   neuron-like Character   N18TG2 x Fischer rat 18 day-embryonic neural retina
Classify   hybrid cell Established by   Nirenberg, M.
Registered by   Nagaoka,A. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum and HAT (100 uM hypoxantine, 1 uM aminopterine, 16 uM thymidine) Methods for Passages   Cells are washed with Ca, Mg-Free PBS when subculture cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J375
Reference
Pubmed id:6149215 Characterization of a glutamic acid neurotransmitter binding site on neuroblastoma hybrid cells.
Malouf AT,Schnaar RL,Coyle JT
J Biol Chem. 1984 Oct 25;259(20):12756-62

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LOT Information

Cell No.   IFO50221 Cell Name   
LOT No.   10252010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medim with HAT (100, 1, 16 uM) and 5% fetal bovine serum (GIBCO Cat. # 10099). Do not add HAT at thawing. Temperature   37 C
Cell Density at Seeding   3.3 - 7.4 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with Ca, Mg-free PBS. (Trypsin treatment is not needed.)
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   85.7 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as hybrid by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50221 Cell Name   N18-RE-105
LOT No.   1030 Lot Specification   distribution
Medium   D.MEM, 95% ; FBS, 5% ; HAT(100, 1, 16uM) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Ca, Mg-Free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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