IFO50289 Becker

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50289 Cell Name   Becker
Profile   astrocytoma, GFAP-negative Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57
Identity   available Tissue for Primary Cancer   neural
Case history   astrocytoma, grade III-IV Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   astrocytoma, GFAP-negative
Classify   tumor Established by   Pfreundschuh, M. et al.
Registered by   Kaji,K. Regulation for Distribution   
Comment    Year   1990
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1093
Reference
Pubmed id:11542786 Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.
Suzuki M,Kase Y,Kanai T,Ando K
Adv Space Res. 1998;22(4):561-8
Pubmed id:283420 Serological analysis of cell surface antigens of malignant human brain tumors.
Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122-6

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LOT Information

Cell No.   IFO50289 Cell Name   Becker
LOT No.   988 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability (%)   85 Antibiotics Used   free
Passage Number   Unknown (>4 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:9
D7S820:11
D16S539:10,14
VWA:16,17
TH01:8,9
AM:X,Y
TPOX:8,11
CSF1PO:12,13
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Additional information   Lot# is labeled as R597F988. Cell# isn't labeled.
Cell No.   IFO50289 Cell Name   Becker
LOT No.   02022017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   23 - 42 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability (%)   94.2 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   
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