IFO50298 Balb/c 3T3 A31-I-1

Cell information

Important Notice(s)

Balb/c 3T3 A31-1に派生する細胞株の由来の問題について

The problem of origin of Balb/c 3T3 A31-1 derived cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50298 Cell Name   Balb/c 3T3 A31-I-1
Profile   fibroblast, subclone of Balb/3T3-A31-1, (Strain problem was found. Refer Important Notice at cell detail page.) Other Name   
Animal   mouse Strain   Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   Origin of this cell line, Balb/c 3T3 A31-1, was found not to be derived from BALB 3T3 A31.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast. This cell line exhibits contact inhibition.
Classify   normal Established by   Kakunaga, T.
Registered by   Tatsuka,M. Regulation for Distribution   
Comment    Year   1990
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J646
Pubmed id:7394516Cell variants showing differential susceptibility to ultraviolet light--induced transformation.
Kakunaga T,Crow JD
Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4306523Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells.
Todaro GJ,Aaronson SA
Virology. 1969 May;38(1):174-9
Pubmed id:4301006Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
J Cell Biol. 1963 May;17():299-313


LOT Information

Cell No.   IFO50298 Cell Name   Balb/c 3T3 A31-I-1
LOT No.   1166 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   3-4 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NP, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information