IFO50313 SNG-M

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50313 Cell Name   SNG-M
Profile   endometrial adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   52
Identity   available Tissue for Primary Cancer   uterus
Case history   endometrial adenocarcinoma Metastasis   Yes
Tissue Metastasized   lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal or spindle Character   endometrial adenocarcinoma
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line does not contain a DNA sequence for E6 oncogene of HPV type 16, 18 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   metastatic lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1707
Reference
Pubmed id:642036Effects of progesterone on human endometrial carcinoma cells in vivo and in vitro.
Ishiwata I,Udagawa Y,Okumura H,Nozawa S
J Natl Cancer Inst. 1978 May;60(5):947-54
Pubmed id:858140Development and characterization of established cell lines from primary and metastatic regions of human endometrial adenocarcinoma.
Ishiwata I,Nozawa S,Inoue T,Okumura H
Cancer Res. 1977 Jun;37(6):1777-85

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LOT Information

Cell No.   IFO50313 Cell Name   SNG-M
LOT No.   1118 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (13 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:8,10,11
D7S820:10,11
D16S539:10,11,12
VWA:17,18,20
TH01:6,7
AM:X
TPOX:8,9
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot no. is labeled as R700F1118.
Images
Cell No.   IFO50313 Cell Name   SNG-M
LOT No.   04122019 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   4.9 x 10^5
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   87.3 Additional information   
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