IFO50315 RMG-I

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50315 Cell Name   RMG-I
Profile   clear cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian clear cell carcinoma Metastasis   Yes
Tissue Metastasized   ascites Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal or spindle Character   clear cell carcinoma
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line does not contain a DNA sequence for E6 oncogene of HPV type 16, 18 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1662
Reference
Pubmed id:3154025Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor.
Nozawa S,Tsukazaki K,Sakayori M,Jeng CH,Iizuka R
Hum Cell. 1988 Dec;1(4):426-35

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LOT Information

Cell No.   IFO50315 Cell Name   
LOT No.   12252012 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.9 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 72 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   67.5 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50315 Cell Name   
LOT No.   07022008 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   1.7 x 10^6 (viable)
Viability at cell freezing (%)   52.4 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50315 Cell Name   RMG-I
LOT No.   1002 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^6
Viability at cell freezing (%)   78 Antibiotics Used   free
Passage Number   Unknown (13 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50315 Cell Name   RMG-I
LOT No.   01212019 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 57 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   IFO50315 Cell Name   RMG-I
LOT No.   12212023 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 17412, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 67 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   89.9 Additional information   
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