-Detailed Information [IFO50363]-

IFO50363 KU812

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50363	Cell Name	KU812
Profile	leukemia, chronic myeloid	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	leukemic cell	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	myeloblast-like	Character	leukemia, chronic myeloid
Classify	tumor	Established by	Kishi, K.
Registered by	Kishi,K.	Regulation for Distribution
Comment		Year	1991
Medium	RPMI1640 medium with 10% fetal bovine serum	Methods for Passages	dilution
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	peripheral blood
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0379
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907311

Reference
Pubmed id:2436686	A monoclonal antibody reacting with human basophils. Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH Blood. 1987 May;69(5):1414-8
Pubmed id:3695564	Induction of differentiation of the human leukemia cell line, KU812. Fischkoff SA,Kishi K,Benjamin WR,Rossi RM,Hoessly MC,Hoxie JA,Seals C,Anderson L,Ishizaka T,Hakimi J Leuk Res. 1987;11(12):1105-13
Pubmed id:3858609	A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Kishi K Leuk Res. 1985;9(3):381-90

Images

LOT Information

07082010
972

Cell No.	IFO50363	Cell Name
LOT No.	07082010	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.7 - 2.5 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture), split ratio 1:4 - 1:6.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	3.5 x 10^6
Viability at cell freezing (%)	90.9	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10 D13S317:8 D7S820:12 D16S539:9 VWA:15 TH01:9 AM:X,Y TPOX:12 CSF1PO:12,13
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50363	Cell Name	KU812
LOT No.	972	Lot Specification	distribution
Medium	RPMI 1640, 90% ; FBS, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	dilution
Doubling Time		Cell Number in Vial (cells/1ml)	7 x 10^5
Viability at cell freezing (%)	80	Antibiotics Used	free
Passage Number	Unknown (9 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), AST
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information