IFO50363 KU812

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50363 Cell Name   KU812
Profile   leukemia, chronic myeloid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   leukemic cell Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   myeloblast-like Character   leukemia, chronic myeloid
Classify   tumor Established by   Kishi, K.
Registered by   Kishi,K. Regulation for Distribution   
Comment    Year   1991
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0379
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907311
Reference
Pubmed id:2436686A monoclonal antibody reacting with human basophils.
Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH
Blood. 1987 May;69(5):1414-8
Pubmed id:3695564Induction of differentiation of the human leukemia cell line, KU812.
Fischkoff SA,Kishi K,Benjamin WR,Rossi RM,Hoessly MC,Hoxie JA,Seals C,Anderson L,Ishizaka T,Hakimi J
Leuk Res. 1987;11(12):1105-13
Pubmed id:3858609A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Kishi K
Leuk Res. 1985;9(3):381-90

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LOT Information

Cell No.   IFO50363 Cell Name   
LOT No.   07082010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.7 - 2.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture), split ratio 1:4 - 1:6.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:12
D16S539:9
VWA:15
TH01:9
AM:X,Y
TPOX:12
CSF1PO:12,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50363 Cell Name   KU812
LOT No.   972 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown (9 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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