IFO50375 MSS31

Cell information

Important Notice(s)

RITC80-7 medium

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50375 Cell Name   MSS31
Profile   spleen stromal cell line, (C57BL/6 mouse) Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   newborn
Identity    Tissue for Primary Cancer   spleen
Case history   spleen stromal cell Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibrlblast-like Character   spleen stromal cell line
Classify    Established by   Yanai, N. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Methods for Passages   25 ug/ml pronase-Hanks'BSS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5561
Reference
Pubmed id:2032312Endothelial cells create a hematopoietic inductive microenvironment preferential to erythropoiesis in the mouse spleen.
Yanai N,Satoh T,Obinata M
Cell Struct Funct. 1991 Feb;16(1):87-93
Pubmed id:2804371Spleen stromal cell lines selectively support erythroid colony formation.
Yanai N,Matsuya Y,Obinata M
Blood. 1989 Nov 15;74(7):2391-7




LOT Information

Cell No.   IFO50375 Cell Name   MSS31
LOT No.   1021 Lot Specification   distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin Temperature   37 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Lot# is labeled as R812F1021
Cell No.   IFO50375 Cell Name   MSS31
LOT No.   08182015 Lot Specification   distribution
Medium   RITC80-7 medium with 2% fetal bovine serum, 10 ng/ml EGF, 5 ug/L monoethanolamine, 10 ug/ml transferrin and 1 ug/ml insulin Temperature   37 C
Cell Density at Seeding   2 - 6 x 10^4 cells/mL Methods for Passages   25 ug/ml pronase in Hanks'balanced salt solution (HBSS)
Doubling Time   approx. 36 hrs Cell Number in Vial (cells/1ml)   8.8 x 10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88
Additional information   
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