IFO50411 A-431

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50411 Cell Name   A-431
Profile   epidermoid carcinoma, high expression of EGF receptor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   epidermoid carcinoma
Case history   epidermoid carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   epidermoid carcinoma, high expression of EGF receptor
Classify   tumor Established by   Giard, D. J. et al.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1993
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   epidermoid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0037
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910925
Reference
Pubmed id:2458340 Differential effects of in vitro mycoplasma infection on interleukin-1 alpha and beta mRNA expression in U937 and A431 cells.
Demczuk S,Baumberger C,Mach B,Dayer JM
J Biol Chem. 1988 Sep 15;263(26):13039-45
Pubmed id:2459591 The epidermal growth factor- and interferon-independent effects of double-stranded RNA in A431 cells.
Chapekar MS,Knode MC,Glazer RI
Mol Pharmacol. 1988 Oct;34(4):461-6
Pubmed id:3526330 yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
Semba K,Nishizawa M,Miyajima N,Yoshida MC,Sukegawa J,Yamanashi Y,Sasaki M,Yamamoto T,Toyoshima K
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5459-63
Pubmed id:4357758 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP
J Natl Cancer Inst. 1973 Nov;51(5):1417-23

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LOT Information

Cell No.   IFO50411 Cell Name   A-431
LOT No.   1165 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   4-7 x 10^4 cells/ml Methods for Passages   trypsin-EDTA, subcultivation ratio ; 1:2
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (4 at IFO) PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NP, G6PD (type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:9,13
D7S820:10
D16S539:12,14
VWA:15,17
TH01:9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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