IFO50416 3T3-L1

Cell information

Important Notice(s)

3T3-L1、登録番号IFO50416とJCRB9014との違い/ Difference in JCRB9014 3T3-L1 and IFO50416 3T3 L1

3T3 L1細胞の解凍、培養に関する注意 / Note for culturing 3T3-L1.

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50416 Cell Name   3T3-L1
Profile   3T3 L1 cell line is a substrain of 3T3-Swiss albino cell line. 3T3 L1 cells convert adipose-like cells at confluent. Other Name   
Animal   mouse Strain   Swiss albino
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   a substrain of 3T3-Swiss albino cell line
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, 3T3-Swiss derivative, adipocyte differentiation
Classify   normal Established by   Green, H. et al.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1993
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0123
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LOT Information

Cell No.   IFO50416 Cell Name   
LOT No.   07232012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine. Temperature   37 C
Cell Density at Seeding   1.8 - 5.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50416 Cell Name   
LOT No.   07032008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   31 hrs Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50416 Cell Name   
LOT No.   12142004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 2.2 days Cell Number in Vial (cells/1ml)   2.6 x 10^5
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50416 Cell Name   
LOT No.   07122001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS(-)
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^5
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50416 Cell Name   3T3-L1
LOT No.   1085 Lot Specification   distribution
Medium   D.MEM, 90% ; calf serum, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^5
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50416 Cell Name   3T3-L1
LOT No.   02202015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine. Temperature   37 C
Cell Density at Seeding   2.7 - 4.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time   D.T. = approx. 54 hrs Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   66.4 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   66.4
Additional information   
Cell No.   IFO50416 Cell Name   3T3-L1
LOT No.   08012016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine. Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.0
Additional information   labeled as 3T3-1 on vials.
Cell No.   IFO50416 Cell Name   3T3-L1
LOT No.   02212018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine. Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.3
Additional information   
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