IFO50431 KP-N-YN

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50431 Cell Name   KP-N-YN
Profile   Alpha smooth muscle actin-positive. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   Yes
Tissue Metastasized   pararenal lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   neuroblastoma, from lymph node, smooth muscle marker-expressing
Classify   tumor Established by   Sugimoto, T. et al.
Registered by   Sugimoto,T. Regulation for Distribution   
Comment    Year   1994
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   neuroblastoma
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1341
Reference
Pubmed id:1329930 [Analysis of surface membrane antigens, cytoskeletal proteins and N-myc oncogene in pediatric solid malignant tumors, their diagnostic usefulness and relevant problems].
Sugimoto T,Matsumura T,Horii Y,Saida T,Hosoi H,Saida T,Mine H,Sawada T
Hum Cell. 1992 Mar;5(1):1-11

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LOT Information

Cell No.   IFO50431 Cell Name   KP-N-YN
LOT No.   1124 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:8,11
D7S820:11,13
D16S539:12
VWA:14,17
TH01:7,9
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Lot# is labeled as R855F1124 on vials.
Cell No.   IFO50431 Cell Name   KP-N-YN
LOT No.   12182017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 60 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^7
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80
Additional information   
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