IFO50434 KALS-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50434 Cell Name   KALS-1
Profile   GFAP is weakly positive. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   74
Identity   available Tissue for Primary Cancer   neural
Case history   glioma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   biporar,polygonal Character   glioma, GFAP, vimentin and CD13-positive
Classify   tumor Established by   Lee, S.
Registered by   Sagawa,K. Regulation for Distribution   
Comment    Year   1994
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1323
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LOT Information

Cell No.   IFO50434 Cell Name   KALS-1
LOT No.   989 Lot Specification   distribution
Medium   RPMI 1640, 95% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^5
Viability (%)   80 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Additional information   The vials are labeled as KALS-1 R876F989.
Cell No.   IFO50434 Cell Name   KALS-1
LOT No.   09142016 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.6 - 2.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability (%)   82 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Additional information   
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