IFO50476 NKM-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50476 Cell Name   NKM-1
Profile   leukemia, acute myeloid, myeloid leukemia cells, responsive to G-CSF and M-CSF for growth Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   myeloid leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   leukemia, acute myeloid, myeloid leukemia cells, responsive to G-CSF and M-CSF for growth
Classify   tumor Established by   Kataoka, T. et al.
Registered by   Ogura,M. Regulation for Distribution   
Comment    Year   1998
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Dilution, split ratio 1:4-1:6
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1607
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LOT Information

Cell No.   IFO50476 Cell Name   
LOT No.   06112004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovien serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2- 4 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   28 hrs Cell Number in Vial (cells/1ml)   5.9 x 10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50476 Cell Name   NKM-1
LOT No.   920 Lot Specification   seed/distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:11,13
D7S820:10,11
D16S539:9,12
VWA:17
TH01:6,9
AM:X
TPOX:9,11
CSF1PO:10
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   IFO50476 Cell Name   NKM-1
LOT No.   05232014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.8 - 5.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:11,13
D7S820:10,11
D16S539:9,12
VWA:17
TH01:6,9
AM:X
TPOX:9,11
CSF1PO:10
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   82
Additional information   
Cell No.   IFO50476 Cell Name   NKM-1
LOT No.   10302018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.3 - 4.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   83.0
Additional information   
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