IFO50488 OUMS-27

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50488 Cell Name   OUMS-27
Profile   chondrosarcoma, type 1 ,2 and 3 collagens; type 9 and 11 collagen mRNA; proteoglycan-producing Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   sarcoma
Case history   chondrosarcoma, grade III tumor Metastasis   
Tissue Metastasized    Genetics   mutation of the p53 gene, heretoploid
Life Span   infinite Crisis PDL   
Morphology   elongated polygonal Character   chondrosarcoma, type 1, 2 and 3 collagens; type 9 and 11 collagen mRNA; proteoglycan-producing
Classify   tumor Established by   Kunisada, T.
Registered by   Namba,M. Regulation for Distribution   
Comment    Year   1998
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage   5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone, left proimal humerus
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested,GAPDH is positive control]
Tissue Type   chondrosarcoma
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3090
Reference
Pubmed id:9714054 A new human chondrosarcoma cell line (OUMS-27) that maintains chondrocytic differentiation.
Kunisada T,Miyazaki M,Mihara K,Gao C,Kawai A,Inoue H,Namba M
Int J Cancer. 1998 Sep 11;77(6):854-9

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LOT Information

Cell No.   IFO50488 Cell Name   
LOT No.   06132008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subculture once a week.
Doubling Time   approx. 75 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^5
Viability (%)   87.5 Antibiotics Used   free
Passage Number   P144 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   IFO50488 Cell Name   
LOT No.   12032002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovien serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   5 x 10^3 cells/cm2 at one week interval Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   67 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability (%)   74.3 Antibiotics Used   free
Passage Number   P144 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by G6PD (type B), NP, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   IFO50488 Cell Name   OUMS-27
LOT No.   1096 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^5
Viability (%)   84 Antibiotics Used   free
Passage Number   P143 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Additional information   
Cell No.   IFO50488 Cell Name   OUMS-27
LOT No.   02242015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.1 - 2.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   87 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability (%)   72.9 Antibiotics Used   free
Passage Number   P144 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:10,12
D16S539:14
VWA:14
TH01:9
AM:X
TPOX:11
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
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