-Detailed Information [IFO50488]-

IFO50488 OUMS-27

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50488	Cell Name	OUMS-27
Profile	chondrosarcoma, type 1 ,2 and 3 collagens; type 9 and 11 collagen mRNA; proteoglycan-producing	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age
Identity	available	Tissue for Primary Cancer	sarcoma
Case history	chondrosarcoma, grade III tumor	Metastasis
Tissue Metastasized		Genetics	mutation of the p53 gene, heretoploid
Life Span	infinite	Crisis PDL
Morphology	elongated polygonal	Character	chondrosarcoma, type 1, 2 and 3 collagens; type 9 and 11 collagen mRNA; proteoglycan-producing
Classify	tumor	Established by	Kunisada, T.
Registered by	Namba,M.	Regulation for Distribution
Comment		Year	1998
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage	5 x 10^5 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	bone, left proimal humerus
Tissue Type	chondrosarcoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3090

Reference
Pubmed id:9714054	A new human chondrosarcoma cell line (OUMS-27) that maintains chondrocytic differentiation. Kunisada T,Miyazaki M,Mihara K,Gao C,Kawai A,Inoue H,Namba M Int J Cancer. 1998 Sep 11;77(6):854-9

Images

Movies

LOT Information

06132008
12032002
1096
02242015
11112019

Cell No.	IFO50488	Cell Name
LOT No.	06132008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	3 - 6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subculture once a week.
Doubling Time	approx. 75 hrs	Cell Number in Vial (cells/1ml)	4.5 x 10^5
Viability at cell freezing (%)	87.5	Antibiotics Used	free
Passage Number	P144	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50488	Cell Name
LOT No.	12032002	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovien serum (FBS lot; BioWhittaker 0S069F)	Temperature	37 C
Cell Density at Seeding	5 x 10^3 cells/cm2 at one week interval	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time	67 hrs	Cell Number in Vial (cells/1ml)	4.2 x 10^5
Viability at cell freezing (%)	74.3	Antibiotics Used	free
Passage Number	P144	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by G6PD (type B), NP, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS-DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50488	Cell Name	OUMS-27
LOT No.	1096	Lot Specification	distribution
Medium	D.MEM, 90% ; FBS, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3 x 10^5
Viability at cell freezing (%)	84	Antibiotics Used	free
Passage Number	P143	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50488	Cell Name	OUMS-27
LOT No.	02242015	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	2.1 - 2.9 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	87 hrs	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	72.9	Antibiotics Used	free
Passage Number	P144	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11 D13S317:12 D7S820:10,12 D16S539:14 VWA:14 TH01:9 AM:X TPOX:11 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	72.9	Additional information

Cell No.	IFO50488	Cell Name	OUMS-27
LOT No.	11112019	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.8 - 5.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 86 hrs.	Cell Number in Vial (cells/1ml)	6.7 x 10^5
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	P144	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	88	Additional information