IFO50491 RNB

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50491 Cell Name   RNB
Profile   RNB cells are the parent cells of MDM2-RNB cells (IFO 50490). Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblastic Character   neonatal rat astrocytes, GFAP-positive
Classify   normal Established by   Kondo, S. and Morimura, T.
Registered by   Morimura,T. Regulation for Distribution   
Comment    Year   1998
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D273
Reference
Pubmed id:8895524 The transforming activities of MDM2 in cultured neonatal rat astrocytes.
Kondo S,Morimura T,Barnett GH,Kondo Y,Peterson JW,Kaakaji R,Takeuchi J,Toms SA,Liu J,Werbel B,Barna BP
Oncogene. 1996 Oct 17;13(8):1773-9




LOT Information

Cell No.   IFO50491 Cell Name   
LOT No.   09212010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   20 hrs. Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50491 Cell Name   RNB
LOT No.   1091 Lot Specification   seed/distribution
Medium   D.MEM, 90% ; inact. FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (14 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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