IFO50492 AM-38

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50492 Cell Name   AM-38
Profile   glioblastoma, GFAP and S-100-positive, ACNU(a cancer chemotherapy drug)-sensitive Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   36
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   glioblastoma, GFAP and S-100-positive, ACNU (a cancer chemotherapy drug)-sensitive
Classify   tumor Established by   Izumi, I. et al.
Registered by   Minemura,K. Regulation for Distribution   
Comment    Year   1998
Medium   Eagle's minimal essential medium with 20% heat-inactivated fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control] Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1070
Reference
Pubmed id:8605218 Quantification of O6-methylguanine-DNA methyltransferase mRNA in human brain tumors.
Mineura K,Watanabe K,Yanagisawa T,Kowada M
Biochim Biophys Acta. 1996 Feb 9;1289(1):105-9
Pubmed id:7947440 Establishment of the two glioma cell lines: YH and AM.
Izumi I,Mineura K,Watanabe K,Kowada M
Hum Cell. 1994 Jun;7(2):101-5
Pubmed id:8145054 Characterization and chemosensitivity of two cell lines derived from human glioblastomas.
Izumu I,Mineura K,Watanabe K,Kowada M
J Neurooncol. 1993 Aug;17(2):111-21

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LOT Information

Cell No.   IFO50492 Cell Name   
LOT No.   12082003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3792782S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 1.6 days Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability (%)   89.6 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   IFO50492 Cell Name   AM-38
LOT No.   961 Lot Specification   seed/distribution
Medium   E.MEM, 80% ; inact. FBS, 20% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability (%)   96 Antibiotics Used   free
Passage Number   Unknown (4 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8
D7S820:12
D16S539:11,13
VWA:17
TH01:7
AM:XY
TPOX:11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Additional information   
Images
Cell No.   IFO50492 Cell Name   AM-38
LOT No.   09082014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability (%)   95 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8
D7S820:12
D16S539:11,13
VWA:17,18,19
TH01:7
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   Ampule was mislabeled as IFO50592. Correct number is IFO50492.
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