IFO50519 A1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50519 | Cell Name | A1 |
|---|---|---|---|
| Profile | derives from p53-deficient mouse brain, used for preparation of conditioned medium for MG5 cells | Other Name | |
| Animal | mouse | Strain | 129/Sv,BDF1,C57BL/6J |
| Genus | Mus | Species | musclus |
| Sex | Age | newborn | |
| Identity | Tissue for Primary Cancer | neural | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Established from p53(-/-) homozygote mice (strain; 129/SvJ, BDF1, C57BL/6J). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | p53-deficient cell line from newborn mouse brain, used for preparation of conditioned medium for MG5. |
| Classify | Established by | Ohsawa, K. | |
| Registered by | Ohsawa,K. | Regulation for Distribution | |
| Comment | This cell line is used for the preparation of conditioned medium for MG5 cells (IFO 50520). Cell type of this cell line has not been identified. | Year | 1999 |
| Medium | Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum. Change medium every 3-4 days. | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:11500035 | Iba1 is an actin-cross-linking protein in macrophages/microglia. Sasaki Y,Ohsawa K,Kanazawa H,Kohsaka S,Imai Y Biochem Biophys Res Commun. 2001 Aug 17;286(2):292-7 |
| Pubmed id:11406294 | Co-induction of argininosuccinate synthetase, cationic amino acid transporter-2, and nitric oxide synthase in activated murine microglial cells. Kawahara K,Gotoh T,Oyadomari S,Kajizono M,Kuniyasu A,Ohsawa K,Imai Y,Kohsaka S,Nakayama H,Mori M Brain Res Mol Brain Res. 2001 Jun 20;90(2):165-73 |
| Pubmed id:10934045 | Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia. Ohsawa K,Imai Y,Kanazawa H,Sasaki Y,Kohsaka S J Cell Sci. 2000 Sep;113 ( Pt 17)():3073-84 |
| Pubmed id:9383038 | Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse. Ohsawa K,Imai Y,Nakajima K,Kohsaka S Glia. 1997 Nov;21(3):285-98 |
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LOT Information
| Cell No. | IFO50519 | Cell Name | |
|---|---|---|---|
| LOT No. | 11132008 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 7 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subcultivate twice a week. |
| Doubling Time | approx. 18 hrs | Cell Number in Vial (cells/1ml) | 4.4 x 10^6 |
| Viability at cell freezing (%) | 93.2 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM (high glucose) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50519 | Cell Name | A1 |
|---|---|---|---|
| LOT No. | 1143 | Lot Specification | distribution |
| Medium | D.MEM (high glucose), 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 5-10 x 10^4 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 | |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50519 | Cell Name | A1 |
|---|---|---|---|
| LOT No. | 06142017 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 0.8 - 1.6 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 23 hrs. | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 |
| Viability at cell freezing (%) | 91.8 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM (high glucose) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91.8 | Additional information |