IFO50525 NCO2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50525 Cell Name   NCO2
Profile   Human myelogenous leukemia cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   35
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic myelogenous lukemia, blast crisis Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Ogura,M,
Registered by   Emi,N. Regulation for Distribution   
Comment    Year   2005
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3043
Pubmed id:1701357 Up-regulation of transferrin receptor gene expression by granulocyte colony-stimulating factor in human myeloid leukemia cells.
Morishita Y,Kataoka T,Towatari M,Ito T,Inoue H,Ogura M,Morishima Y,Saito H
Cancer Res. 1990 Dec 15;50(24):7955-61


LOT Information

Cell No.   IFO50525 Cell Name   NCO2
LOT No.   01132005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   1.2 days Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability (%)   86.3 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Additional information