JCRB0004 A-431

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0004 Cell Name   A-431
Profile   epidermoid carcinoma, high expression of EGF-receptor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   85
Identity   available Tissue for Primary Cancer   epidermoid carcinoma
Case history   established from solid tumor Metastasis   No
Tissue Metastasized    Genetics   epidermoid carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   epidermoid carcinoma
Classify   tumor Established by   Giard,D.J. et al.
Registered by    Regulation for Distribution   No regulations.
Comment    Year   1985
Medium   Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/3 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   epidermoid carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0037
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910925
Reference
Pubmed id:2458340 Differential effects of in vitro mycoplasma infection on interleukin-1 alpha and beta mRNA expression in U937 and A431 cells.
Demczuk S,Baumberger C,Mach B,Dayer JM
J Biol Chem. 1988 Sep 15;263(26):13039-45
Pubmed id:2459591 The epidermal growth factor- and interferon-independent effects of double-stranded RNA in A431 cells.
Chapekar MS,Knode MC,Glazer RI
Mol Pharmacol. 1988 Oct;34(4):461-6
Pubmed id:3526330 yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
Semba K,Nishizawa M,Miyajima N,Yoshida MC,Sukegawa J,Yamanashi Y,Sasaki M,Yamamoto T,Toyoshima K
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5459-63
Pubmed id:4357758 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP
J Natl Cancer Inst. 1973 Nov;51(5):1417-23

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LOT Information

Cell No.   JCRB0004 Cell Name   
LOT No.   03022010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose: 4.5 g/L) with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.2 - 1.6 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 36 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   88.7 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0004 Cell Name   
LOT No.   08052004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   4-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS (Cells will be strongly adhere to substratum. Incubation at 37 C is reccomended.)
Doubling Time   ca 42 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   78.9 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   071696 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:9,13
D7S820:10
D16S539:12,14
VWA:15,17
TH01:9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   042294 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's minimal medium with high glucose and 10% fetal bovine serum(Hyclone) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   Wash with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-10 min.
Doubling Time   Td.=21.0 hrs Cell Number in Vial (cells/1ml)   1.71x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P35 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD(not HeLa type), NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   060293 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (glucose 4.5g/L) with 10% fetal bovine serum (Hyclone 1115975) Temperature   37 C
Cell Density at Seeding   7-8x10^5 cells/100mm dish Methods for Passages   Treat cells by 0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   P32 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   051391 Lot Specification   distribution
Medium   DMEM with 4.5g/l glucose + FCS 10% Temperature   37 C
Cell Density at Seeding   1/3-1/8 dilution Methods for Passages   0.02 % EDTA and 0.25% trypsin treatment.
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P34 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   041790 Lot Specification   distribution
Medium   DMEM with 4.5 g/L glucose + FCS 10 % Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   041388 Lot Specification   distribution
Medium   DMEM with 4.5 g/L glucose + FCS 10 % Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   D.T.=31.0hr Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   030386 Lot Specification   distribution
Medium   DMEM with 4.5 g/L glucose + FCS 10 % Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   04152016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.5 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:9,13
D7S820:10
D16S539:12,14
VWA:15,17
TH01:9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98
Additional information   
Cell No.   JCRB0004 Cell Name   A-431
LOT No.   12132019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98
Additional information   
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