JCRB0007 Pt K2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0007 Cell Name   Pt K2
Profile   DNA replication study Other Name   NBL-5
Animal   marsupial (potoroo, rat kangar Strain   
Genus   Potorous Species   tridactylis
Sex   M Age   adult
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   PtK2 was used in study of chr.DNA replication.
Classify   unknown Established by   Walen,K.H.
Registered by   Takeuchi,M. Regulation for Distribution   Not regulated
Comment   The cell line may be used for the study of keratin fiber. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/2 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0514
Reference
Pubmed id:14337764SPATIAL RELATIONSHIPS IN THE REPLICATION OF CHROMOSOMAL DNA.
WALEN KH
Genetics. 1965 Jun;51():915-29

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LOT Information

Cell No.   JCRB0007 Cell Name   Pt K2
LOT No.   02142005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^5
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0007 Cell Name   Pt K2
LOT No.   071291 Lot Specification   distribution
Medium   MEM + FCS (10%) Temperature   37 C
Cell Density at Seeding   1/2 - 1/3 Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.9x10^5
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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