JCRB0009 P3/NS1/1-Ag4-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0009 Cell Name   P3/NS1/1-Ag4-1
Profile   Mouse plasmacytoma cell line resistant to 8-azaguanine. Other Name   NS1
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   resistant to 10^-4 M 8-azaguanine(not grow in HAT)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   non-secreting mouse myeloma (k-chain synthesized)
Classify   transformed Established by   Kohler,G. & Milstein,C.
Registered by   Sawada,J. Regulation for Distribution   Not regulated
Comment   Used for the preparation of hybridoma. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Subculture twice a week.
Cell Number on Passage   1-2x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   plasmacytoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2155
Reference
Pubmed id:825377Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
Köhler G,Milstein C
Eur J Immunol. 1976 Jul;6(7):511-9
Pubmed id:61874Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity.
Eichmann K
Eur J Immunol. 1975 Aug;5(8):511-7
Pubmed id:4449137Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA.
Cowan NJ,Secher DS,Milstein C
J Mol Biol. 1974 Dec 25;90(4):691-701
Pubmed id:5439579Mouse myelomas and lymphomas in culture.
Horibata K,Harris AW
Exp Cell Res. 1970 Apr;60(1):61-77

Images



LOT Information

Cell No.   JCRB0009 Cell Name   P3/NS1/1-Ag4-1
LOT No.   022594 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRS.DO10400, JRH.1B10062) Temperature   37 C
Cell Density at Seeding   2-5x10^5 cells/ml Methods for Passages   dilution (2-5x10^5 cells/ml)
Doubling Time   T Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   82.1 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0009 Cell Name   P3/NS1/1-Ag4-1
LOT No.   052688 Lot Specification   distribution
Medium   RPMI1640 + FCS 10 % Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Subculture twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0009 Cell Name   P3/NS1/1-Ag4-1
LOT No.   021886 Lot Specification   distribution
Medium   RPMI1640 + FCS 10 % Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Subculture twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   88% Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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