JCRB0017 P388

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0017 Cell Name   P388
Profile   MCA induced lymphoid mouse neoplasma cell line. Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   MCA induced lymphoid neoplasma Metastasis   
Tissue Metastasized    Genetics   Low level IL1 production
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   This cell line is used in testing for antitumor activity by NCI.
Classify   tumor Established by   Dawe,C.J. & Potter,M.
Registered by   Sawada,J. Regulation for Distribution   
Comment   Target cell by cytotoxic T lymphocyte. Year   1985
Medium   RPMI1640 meium with 5x10^-5 M 2-Mercapthoethanole and 10% fetal calf serum Methods for Passages   Dilute twice a week.
Cell Number on Passage   5x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   lymphoid neoplasma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_7222
Reference
Pubmed id:14258726METABOLIC CONTROLS IN CULTURED MAMMALIAN CELLS.
EAGLE H
Science. 1965 Apr 2;148(3666):42-51
Pubmed id:13541471Adaptation to serum-free medium by a phagocytic cell strain derived from a murine lymphoma.
RABSON AS,LEGALLAIS FY,BARON S
Nature. 1958 May 10;181(4619):1343

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LOT Information

Cell No.   JCRB0017 Cell Name   
LOT No.   06252002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum and 50 uM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding   1.5-2.0 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   6.4 x 10^6
Viability at cell freezing (%)   78 Antibiotics Used   free
Passage Number   Unknown (P8 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0017 Cell Name   P388
LOT No.   072594 Lot Specification   distribution
Medium   RPMI1640 with 5x10^(-5M) 2-mercaptoethanol and 10% heat-inactivated fetal bovine serum(JRS. DO10400) Temperature   37 C
Cell Density at Seeding   1-3 x 10^5 cells/ml Methods for Passages   Harvested cells were simply suspended into fresh medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^7
Viability at cell freezing (%)   63.0 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0017 Cell Name   P388
LOT No.   061391 Lot Specification   distribution
Medium   RPMI1640 with 5x10^-5 M 2 ME + FCS 10% Temperature   37 C
Cell Density at Seeding   5.4x10^4 cells/ml Methods for Passages   Dilution twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0017 Cell Name   P388
LOT No.   052587 Lot Specification   distribution
Medium   RPMI1640 w/ 5x10^-5 M 2ME + FCS 10% Temperature   37
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   Dilute twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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