JCRB0019 K562

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0019 Cell Name   K562
Profile   Highly undifferentiated and granurocytic myelogenous leukemia cell line from blast crisis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   53
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic myelogenous leukemia from blast crisis Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Differentiate into erythrocytic, granurocytic & monocytic cells.
Classify   tumor Established by   Lozzio,C.B. & Lozzio,B.B.
Registered by   Taniyama,T. Regulation for Distribution   Not required.
Comment   Differentiation with NaB was tested by Riken Cell Bank (see picture, others(5)). Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Dilution.
Cell Number on Passage   2x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   Highly undifferentiated and granurocytic cell
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0004
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Reference
Pubmed id:11504883 Enhancer activity of HS2 of the human beta-LCR is modulated by distance from the key nucleosome.
Onishi Y,Kiyama R
Nucleic Acids Res. 2001 Aug 15;29(16):3448-57
Pubmed id:3032411 DNA-mediated transfer and cloning of a human multidrug-resistant gene of adriamycin-resistant myelogenous leukemia K562.
Sugimoto Y,Tsuruo T
Cancer Res. 1987 May 15;47(10):2620-5
Pubmed id:6192693 Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:95354 K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Rutherford TR,Clegg JB,Weatherall DJ
Nature. 1979 Jul 12;280(5718):164-5
Pubmed id:570644 Induction of erythroid differentiation in the human leukaemia cell line K562.
Andersson LC,Jokinen M,Gahmberg CG
Nature. 1979 Mar 22;278(5702):364-5
Pubmed id:271272 Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
Collins SJ,Gallo RC,Gallagher RE
Nature. 1977 Nov 24;270(5635):347-9
Pubmed id:163658 Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
Lozzio CB,Lozzio BB
Blood. 1975 Mar;45(3):321-34

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LOT Information

Cell No.   JCRB0019 Cell Name   
LOT No.   08212012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Dilution. Some cells may loosely attach to substratum, and they should be detached by pipetting. Passage twice a week.
Doubling Time   approx. 27 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   87.5 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:9,11
D16S539:11,12
VWA:16
TH01:9.3
AM:X
TPOX:8,9
CSF1PO:9,10
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   
LOT No.   04022009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   1.1 - 1.6 x 10^5 cells/ml Methods for Passages   Dilution. Some cells may loosely attach to substratum, and they should be detached by pipetting. Passage twice a week.
Doubling Time   approx. 21 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   
LOT No.   12062004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   18.6 hrs Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P10 at cellbank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   
LOT No.   07262002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   8.2 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (9 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by G6PD(B), NP, LD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   070196 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0019 Cell Name   K562
LOT No.   111595 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (Flow 0070893) Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0019 Cell Name   K562
LOT No.   092993 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (heat inactivated) (JRH Biosciences lot.1B1062) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   Dilution
Doubling Time   24hrs Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   83.6 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), LD, PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   051392 Lot Specification   distribution
Medium   RPMI1640(Nissui,521109) + F.B.S.10%(J.R.ScientificDO10400) Temperature   37C
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   Dilution
Doubling Time   23Hrs Cell Number in Vial (cells/1ml)   9.6x10^5
Viability at cell freezing (%)   90% Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   080691 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   2-10x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   99.1 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   102990 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   020590 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   030189 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum. Temperature   37
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0019 Cell Name   K562
LOT No.   070387 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   
LOT No.   110298 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   7.5 x 10^4 cells/ml Methods for Passages   Dilution
Doubling Time   ca 20 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   67.0 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0019 Cell Name   K562
LOT No.   06212017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   90.2 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.2
Additional information   
Images
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