JCRB0031 CCRF-HSB2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0031 Cell Name   CCRF-HSB2
Profile   Human T-lymphoblastic leukemia cell line from implanted tumor in Syrian hamster. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   11.5
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute lymphoblastic leukemia. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cultured in vitro from implanted tumor in Syrian hamster.
Classify   tumor Established by   Foley,G.E.
Registered by   Sofuni,T. Regulation for Distribution   Free
Comment   After CCRF-SB was transplanted to new born Syrian hamster, cells were then transferred in vitro culture system again at 8 th transplant. STR-PCR showed the close similarity with the CCRF-SB. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Dilution.
Cell Number on Passage   5x10^5 cells/ml Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   peripheral blood (buffy coat)
Tissue Type   T lymphoblastoid leukemia

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1859
Reference
Pubmed id:4991454The question of stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia.
Adams RA,Pothier L,Flowers A,Lazarus H,Farber S,Foley GE
Exp Cell Res. 1970 Sep;62(1):5-10
Pubmed id:4872716Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2.
Adams RA,Flowers A,Davis BJ
Cancer Res. 1968 Jun;28(6):1121-5
Pubmed id:4170381Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma.
Adams RA
Cancer Res. 1967 Dec;27(12):2479-82

Images



LOT Information

Cell No.   JCRB0031 Cell Name   CCRF-HSB2
LOT No.   090792 Lot Specification   distribution
Medium   RPMI1640 medium (lot.070892,081792) + fetal bovine serum (Flow lab.0070893, Hyclone 1115103) Temperature   37 C
Cell Density at Seeding   1.3x10^4 cells/ml Methods for Passages   Dilution is appropriate for the subculture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0031 Cell Name   CCRF-HSB2
LOT No.   042091 Lot Specification   distribution
Medium   RPMI1640 + FBS 10% Temperature   37
Cell Density at Seeding   1.1x10^5 cells/ml Methods for Passages   dilution
Doubling Time   24 hr Cell Number in Vial (cells/1ml)   10^6 cells
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0031 Cell Name   CCRF-HSB2
LOT No.   052987 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0031 Cell Name   CCRF-HSB2
LOT No.   042191 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum. Lot # and passage # that printed on ampoule are wrong (mislabeled). Correctly the lot# is 042191 and passage # is P5* as shown in the data sheet. Temperature   37 C
Cell Density at Seeding   1.1 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0031 Cell Name   CCRF-HSB2
LOT No.   08272021 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.00-2.00x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.22x10^6
Viability at cell freezing (%)   98.05 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:10,12
D7S820:11,12
D16S539:9,13
VWA:18,19
TH01:9,10
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.96
Additional information   
Images
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