JCRB0035 RPMI 1788

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0035 Cell Name   RPMI 1788
Profile   established from normal blood, IgM secreting Other Name   RPMI1788
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   33
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   normal male Metastasis   
Tissue Metastasized    Genetics   normal
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   IgM secreting(light chain), positive for HLA2.7
Classify   transformed Established by   Moore,G. et al.
Registered by   Sofuni,T. Regulation for Distribution   free
Comment    Year   
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   over 5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Virus Summary   tested Virus Tested   CMV(-), EBV(+), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2710
Reference
Pubmed id:3485474 Partial purification and characterization of a recombinase from human cells.
Hsieh P,Meyn MS,Camerini-Otero RD
Cell. 1986 Mar 28;44(6):885-94
Pubmed id:4683261 Effect on growth but not on chromosomes of the mammalian cells after treatment with three organophosphorus insecticides.
Huang CC
Proc Soc Exp Biol Med. 1973 Jan;142(1):36-40
Pubmed id:5353241 Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies.
Huang CC,Moore GE
J Natl Cancer Inst. 1969 Nov;43(5):1119-28

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LOT Information

Cell No.   JCRB0035 Cell Name   RPMI 1788
LOT No.   073192 Lot Specification   distribution
Medium   RPMI1640 (lot.070892) + fetal calf serum 10% (FlowLab.0070893) Temperature   37 C
Cell Density at Seeding   1.3x10^6 cells/ml Methods for Passages   dilution, 2-3 folds dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   88.4 Antibiotics Used   free
Passage Number   P5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0035 Cell Name   RPMI 1788
LOT No.   091389 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   over 5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0035 Cell Name   RPMI 1788
LOT No.   041086 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   over 5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0035 Cell Name   RPMI 1788
LOT No.   02272014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^5 cells/mL Methods for Passages   Simple dilution.
Doubling Time    Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10%DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.1
Additional information   
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