JCRB0054 LU65A

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0054 Cell Name   LU65A
Profile   giant cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Suzuki,K. et al.
Registered by   Suzuki,K. Regulation for Distribution   Permission for the distribution required.
Comment    Year   5
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   giant cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3012
Reference
Pubmed id:2112525A simple, general method for detecting retroviral RNAs expressed in cells.
Harada F,Hirose Y,Tanaka M,Sasaki T,Kamiya H,Miura K
Jpn J Cancer Res. 1990 Mar;81(3):232-7
Pubmed id:2659722Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells.
Suzuki K,Miyasaka H,Ota H,Yamakawa Y,Tagawa M,Kuramoto A,Mizuno S
J Exp Med. 1989 Jun 1;169(6):1895-901
Pubmed id:3935627Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.
Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N
Jpn J Cancer Res. 1985 Oct;76(10):967-76

Images



LOT Information

Cell No.   JCRB0054 Cell Name   LU65A
LOT No.   040795 Lot Specification   FDSC/distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Bio Cell, 4001776). Lot # labeled on Ampoule is wrong. Correct lot # is 040795. Temperature   37 C
Cell Density at Seeding   7.8 - 17.9x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA mix.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.5x10^5
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   P14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Ampoules were printed 040759 by mistake. 040795 is correct.
Cell No.   JCRB0054 Cell Name   LU65A
LOT No.   111789 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.05% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0054 Cell Name   LU65A
LOT No.   102885 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.05% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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