JCRB0060 LLC-PK1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0060 Cell Name   LLC-PK1
Profile   susceptible to rubella, vaccinia and herpes viruses Other Name   
Animal   pig Strain   
Genus   Sus Species   scrofa
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   susceptible to viruses, rubella,vaccinia & herpes.
Classify   transformed Established by   Hull,R.N. et al.
Registered by   Suzuki,K. Regulation for Distribution   The cell line was originally deposited to ATCC forthe patenting purpose.
Comment    Year   1985
Medium   Medium 199 with 3% fetal calf serum Methods for Passages   Treat cells with 0.02% EDTA and 0.1% trypsin. Passage cells every 4 days.
Cell Number on Passage   10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0391
Reference
Pubmed id:6243876 Studies of renal cell function using cell culture techniques.
Handler JS,Perkins FM,Johnson JP
Am J Physiol. 1980 Jan;238(1):F1-9
Pubmed id:6255473 Insulin and serum increase the number of receptors for vasopressin in a kidney-derived line of cells grown in a defined medium.
Roy C,Preston AS,Handler JS
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5979-83
Pubmed id:18812 Studies on tissue culture produced plasminogen activator. I. Preliminary observations and the enhancing effect of colchicine and other antimitotic agents.
Hull RN,Cherry WR,Huseby RM,Clavin SA
Thromb Res. 1977 May;10(5):669-77
Pubmed id:828141 The origin and characteristics of a pig kidney cell strain, LLC-PK.
Hull RN,Cherry WR,Weaver GW
In Vitro. 1976 Oct;12(10):670-7

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LOT Information

Cell No.   JCRB0060 Cell Name   
LOT No.   06012007 Lot Specification   distribution
Medium   Medium 199 with 3% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (7 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as pig by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Medium 199 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0060 Cell Name   
LOT No.   05182001 Lot Specification   distribution
Medium   Medium 199 with 3% fetal calf serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as pig by NP, G6PD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0060 Cell Name   LLC-PK1
LOT No.   080996 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0060 Cell Name   LLC-PK1
LOT No.   100692 Lot Specification   distribution
Medium   Medium 199 (072992, 090992) with 3% fetal bovine serum (Flow Lab.0070893) Temperature   37 C
Cell Density at Seeding   9.7x10^4 cell/ml Methods for Passages   Cells are washed once with PBS, then treated with 0.25%trypsin and 0.02%EDTA at 37C for 10min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.68x10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=39 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0060 Cell Name   LLC-PK1
LOT No.   012689 Lot Specification   distribution
Medium   Medium 199 with 3% fetal calf serum Temperature   37 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   0.02% EDTA and 0.1% trypsin. Passage every 4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0060 Cell Name   LLC-PK1
LOT No.   09222017 Lot Specification   distribution
Medium   Medium 199 with 3% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.3 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   7.7 x 10^6
Viability at cell freezing (%)   89.8 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Medium 199 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.8
Additional information   
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