JCRB0061 AZ-521

Cell information

Important Notice(s)

JCRB0061 AZ-521細胞の由来について

JCRB0061 AZ-521: the problem of origin

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0061 Cell Name   AZ-521
Profile   Reported as Human gastric cancer cell line. The STR DNA analysis pattern of AZ-521 revealed to be identical to that of other cell line derived from human duodenum carcinoma, HuTu 80 in ATCC (registry number, HTB-40) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   stomach
Case history   gastric cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Reported as Human gastric cancer cell line. The STR DNA analysis pattern of AZ-521 revealed to be identical to that of other cell line derived from human duodenum carcinoma, HuTu 80 in ATCC (registry number, HTB-40)
Classify   tumor Established by   
Registered by   Akiyama,M. Regulation for Distribution   !! the problem of origin exists !!
Comment   Mycoplasma contamination was detected when submitted to NIHS and eliminated at 1986. Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/4 Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2862
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907073
Reference
Pubmed id:1406053 Regulation of arginase production by glucocorticoid in three human gastric cancer cell lines.
Wu CW,Wang SR,Chien SL,Yeh TH,Lian SL,Shimizu N,Lui WY,P'eng FK,Chi CW
Life Sci. 1992;51(17):1355-61
Pubmed id:2706690 Nuclear granules recognized by some monoclonal antibodies against intermediate filament protein locate on chromosomes during mitosis.
Kamei H
Cell Biol Int Rep. 1989 Mar;13(3):291-300
Pubmed id:2736210 Production of transforming growth factor-alpha in human tumour cell lines.
Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K
Br J Cancer. 1989 May;59(5):761-5

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LOT Information

Cell No.   JCRB0061 Cell Name   
LOT No.   11162006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.5 - 1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0061 Cell Name   AZ-521
LOT No.   063095 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (JRS Temperature   37 C
Cell Density at Seeding   2.1x10^4 cell/sq.cm. Methods for Passages   Harvest cells with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.67x10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   under investigation
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:9,11
D16S539:10,11
VWA:16,18
TH01:7
AM:XY
TPOX:9,11
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0061 Cell Name   AZ-521
LOT No.   060392 Lot Specification   distribution
Medium   MEM(Nissui,302111) + non essential a.a.(GIBUCO,liqid,Control#11p2201) + F.B.S.10%(DO10400) Temperature   37C
Cell Density at Seeding   3x10^4 cells/cm^2 Methods for Passages   0.25% Trypsin + 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.46x10^6
Viability at cell freezing (%)   88% Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0061 Cell Name   AZ-521
LOT No.   020988 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   83% Antibiotics Used   free
Passage Number   P14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0061 Cell Name   AZ-521
LOT No.   05282014 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   6.1 x 10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:9,11
D16S539:10,11
VWA:16,18
TH01:7
AM:X,Y
TPOX:9,11
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.5
Additional information   
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